Analysis of Sertoli Cell-Secreted Proteins by Two-Dimensional Gel Electrophoresis

Analysis of Sertoli cell-secreted proteins by two-dimensional gel electrophoresis.

The [35S]methionine-labeled proteins secreted from cultured Sertoli cells were analyzed by two-dimensional gel electrophoresis and fluorography. Major polypeptides which were resolved by this procedure were designated by number and further analyzed. Many of these major polypeptides appeared as a series of spots which corresponded to charge isomers. Two of these polypeptides (5 and 6) were shown...

Two dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines.

An improved method of two-dimensional gel electrophoresis has been developed which produces high resolution, reproducible images suitable for computer analysis. In the images that are presented, more than 800 proteins have been resolved without significant overlap, and many more proteins can be detected after longer exposures. To establish the usefulness of such methods for detailed quantitativ...

Two-dimensional Gel Electrophoresis

Analysis of triplet repeat replication by two-dimensional gel electrophoresis.

Expansions of triplet repeats are responsible for more than 15 hereditary neurological disorders in humans. Triplet repeats are fairly stable when the number of elementary units is under approx 30, but become polymorphic in length with a clear bias for expansions when this threshold is exceeded. This results in the rapid addition of hundreds or even thousands of extra repeats and, ultimately, d...

Identification by Two-Dimensional Gel Electrophoresis

We have previously identified proteins in fractions of culture filtrate ofMycobacterium tuberculosis with the capacity to induce cytokine production in monocytes, by using a technique we defined as "monocyte Western blotting" (immunoblotting). In this series of experiments, we have extended this technique to two-dimensional gel electrophoresis and have identified a novel 58-kDa protein ofM. tub...